Amy Rosenzweig Professor

Research Summary:

The Rosenzweig group is focused on understanding metalloprotein function on the molecular level. We use structural, spectroscopic, biochemical, genetic, and bioinformatic approaches to attack problems at the forefront of bioinorganic chemistry. Specific areas of interest include biological methane oxidation, oxygen activation of metalloenzymes, metal uptake and transport, and natural products biosynthesis.

Selected Publications:

Cell-Free Protein Synthesis of Particulate Methane Monooxygenase into Nanodiscs. Koo CW, Hershewe JM, Jewett MC, Rosenzweig AC. ACS Synth Biol. 2022 Dec 16;11(12):4009-4017. doi: 10.1021/acssynbio.2c00366. Epub 2022 Nov 23. PMID: 36417751; PMCID: PMC9910172.

Structure and activity of particulate methane monooxygenase arrays in methanotrophs. Zhu Y, Koo CW, Cassidy CK, Spink MC, Ni T, Zanetti-Domingues LC, Bateman B, Martin-Fernandez ML, Shen J, Sheng Y, Song Y, Yang Z, Rosenzweig AC, Zhang P. Nat Commun. 2022 Sep 5:13(1):5221. doi: 10.1038/s41467-022-32752-9. PMID: 36064719; PMCID: PMC9445010.

A mixed-valent Fe(II)Fe(III) species converts cysteine to an oxazolone/thioamide pair in methanobactin biosynthesis. Park YJ, Jodts RJ, Slater JW, Reyes RM, Winton VJ, Montaser RA, Thomas PM, Dowdle WB, Ruiz A, Kelleher NL, Bollinger JM Jr, Krebs C, Hoffman BM, Rosenzweig AC. Proc Natl Acad Sci U S A. 2022 Mar 29;119(13):e2123566119. doi: 10.1073/pnas.2123566119. Epub 2022 Mar 23. PMID: 35320042; PMCID: PMC9060507.

Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer. Koo, CW, Tucci FJ, He Y, Rosenzweig AC. Science. 2022 Mar 18;375(6586):1287-1291. doi: 10.1126/science.abm3282. Epub 2022 Mar 17. PMID: 35298269; PMCID: PMC9357287.

Characterization of a Copper-Chelating Natural Product from the Methanotroph Methylosinus sp. LW3. Park YJ, Roberts GM, Montaser R, Kenney GE, Thomas PM, Kelleher NL, Rosenzweig AC. Biochemistry. 2021 Sep 28;60(38):2845-2850. doi: 10.1021/acs.biochem.1c00443. Epub 2021 Sep 12. PMID: 34510894; PMCID: PMC8739258.

Coordination of the Copper Centers in Particulate Methane Monooxygenase: Comparison between Methanotrophs and Characterization of the CuC Site by EPR and ENDOR Spectroscopies. Jodts RJ, Ross MO, Koo CW, Doan PE, Rosenzweig AC, Hoffman BM. J Am Chem Soc. 2021 Sep 22;143(37):15358-15368. doi: 10.1021/jacs.1c07018. Epub 2021 Sep 9. PMID: 34498465; PMCID: PMC8811777.

Copper binding by a unique family of metalloproteins is dependent on kynurenine formation. Manesis AC, Jodts RJ, Hoffman BM, Rosenzweig AC. Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):e2100680118. doi: 10.1073/pnas.2100680118. PMID: 34074779; PMCID: PMC8201829.

Particulate methane monooxygenase contains only mononuclear copper centers. Ross MO, MacMillan F, Wang J, Nisthal A, Lawton TJ, Olafson BD, Mayo SL, Rosenzweig AC, Hoffman BM. Science. 2019 May 10;364(6440):566-570. doi: 10.1126/science.aav2572. PMID: 31073062; PMCID: PMC6664434.

The biosynthesis of methanobactin. Kenney GE, Dassama LMK, Pandelia ME, Gizzi AS, Martinie RJ, Gao P, DeHart CJ, Schachner LF, Skinner OS, Ro SY, Zhu X, Sadek M, Thomas PM, Almo SC, Bollinger JM Jr, Krebs C, Kelleher NL, Rosenzweig AC. Science. 2018 Mar 23;359(6382):1411-1416. doi: 10.1126/science.aap9437. PMID: 29567715; PMCID: PMC5944852.

Selected Honors:

The Protein Society Hans Neurath Award, 2021

American Chemical Society Alfred Bader Award in Bioinorganic or Bioorganic Chemistry, 2021

Elected Member, National Academy of Sciences, 2017

Elected Fellow, American Academy of Arts and Sciences, 2014

Royal Society of Chemistry Joseph Chatt Award, 2014

American Chemical Society Nobel Laureate Signature Award for Graduate Education, 2006

MacArthur Fellow, 2003