Robert Holmgren Professor; Director, Program in Biological Sciences

Research Summary:

Our laboratory studies Hedgehog signal transduction, which plays a central role in animal development and human disease.  The main focus of the lab is the identification and characterization of new pathway components.  Our approach is to use an in vivo RNAi suppressor/enhancer screen to discover candidate genes, which are then validated and studied to determine how they function within the pathway. 

Selected Publications:

Dose-dependent transduction of Hedgehog relies on phosphorylation-based feedback between the G-protein-coupled receptor Smoothened and the kinase Fused. Sanial M, Bécam I, Hofmann L, Behague J, Argüelles C, Gourhand V, Bruzzone L, Holmgren RA, Plessis A. Development. 2017 May 15;144(10):1841-1850. 

Control of the dynamics and homeostasis of the Drosophila Hedgehog receptor Patched by two C2-WW-HECT-E3 Ubiquitin ligases. Brigui A, Hofmann L, Argüelles C, Sanial M, Holmgren RA, Plessis A. Open Biology. 2015; 5(10):150112. 

Chemical map of Schizosaccharomyces pombe reveals species-specific features in nucleosome positioning. Moyle-Heyrman G, Zaichuk T, Xi L, Zhang Q, Uhlenbeck OC, Holmgren R, Widom J, Wang JP. Proc Natl Acad Sci USA. 2013 Dec 10; 110(50):20158-63. 

Specific Inhibition of the Transcription Factor Ci by a Cobalt(III) Schiff Base-DNA Conjugate. Hurtado RR, Harney AS, Heffern MC, Holbrook RJ, Holmgren RA, and Meade TJ. Molecular Pharmaceutics. 2012 February 6;9(2):325-333.

A Three-part Signal Governs Differential Processing of Gli1 and Gli3 Proteins by the Proteasome. Schrader EK, Harstad KG, Holmgren RA, and Matouschek A. Journal of Biological Chemistry. 2011 November 11;286(45):39051-39058.

Regulation of Ci and Su(fu) nuclear import in Drosophila. Sisson BE, Ziegenhorn SL, and Holmgren RA. Developmental Biology. 2006 June 1;294(1):258-270.

Regulation of the Drosophila transcription factor, Cubitus interruptus, by two conserved domains. Croker JA, Ziegenhorn SL, and Holmgren RA. Developmental Biology. 2006 March 15;291(2):368-381.

A conserved processing mechanism regulates the activity of transcription factors Cubitus interruptus and NF-kB. Tian L, Holmgren RA, and Matouschek, A. Nature Structural & Molecular Biology. 2005 December;12(12):1045-1053.